Mouse Agouti Related Protein (AGRP) ELISA Kit

In metabolic and neuroscience research, Agouti-related protein (AGRP) is recognized as a key orexigenic neuropeptide produced in the arcuate nucleus of the hypothalamus, where it functions as an endogenous antagonist at melanocortin-3 and melanocortin-4 receptors (MC3R/MC4R). By blocking melanocortin signaling, AGRP stimulates appetite and promotes weight gain, making it a central biomarker for studies of obesity, energy balance, and feeding behavior in mouse models.

The Assay Genie Mouse AGRP ELISA Kit is purpose-built for quantifying endogenous mouse AGRP in tissue homogenates, cell lysates, and blood-derived specimens using a quantitative colorimetric method in a 96-assay microplate format. The assay is reactive specifically with mouse samples, supporting investigation of leptin resistance, hypothalamic signaling, and melanocortin pathway pharmacology in murine systems.

Each kit provides a pre-coated microplate and reagents sufficient for 96 assays, intended for in vitro research use only. Store reagents according to the supplied insert to preserve antibody reactivity across freeze-thaw cycles, and process samples in duplicate for accurate quantification of this appetite-regulating peptide in hypothalamic tissue extracts and cultured neuronal preparations.