Mouse AFGF/FGF1 (Acidic Fibroblast Growth Factor 1) ELISA Kit (MOES01694)

This quantitative immunoassay kit from Assay Genie enables the in vitro measurement of acidic fibroblast growth factor 1 (FGF1), also referred to as AFGF or aFGF, in mouse specimens. The kit provides reagents for 96 assays and is intended for research use only, supporting studies in angiogenesis, wound healing, cardiac development, and tumor biology where FGF1 signaling plays a documented role.

Mouse AFGF/FGF1 circulates at low endogenous levels, and researchers commonly employ this kit to compare healthy versus diseased cohorts, including tumor xenograft models, diabetic wound tissues, and ischemic cardiac tissue lysates. Specimens are typically prepared by centrifugation of collected blood to isolate the liquid fraction, after which they are added directly to pre-coated wells. The protocol accommodates a small sample volume per well, and absorbance readings are used to determine FGF1 concentration against a standard curve.

Provided reagents are sufficient for one full microplate run, with all buffers, standards, and detection components supplied ready to use. The kit is configured for mouse reactivity and is not validated for other species. Storage recommendations and reagent stability are detailed in the enclosed product manual.