Mouse Adrenocorticotropic Hormone (ACTH) ELISA Kit

This 96-assay microplate immunoassay from Assay Genie quantifies mouse adrenocorticotropic hormone (ACTH), a 39-amino acid polypeptide released by the anterior pituitary that drives glucocorticoid production through the hypothalamic-pituitary-adrenal axis.

The Mouse Adrenocorticotropic Hormone (ACTH) ELISA Kit from Assay Genie is configured for in vitro research use only and supports studies of stress response, circadian rhythmicity, adrenal regulation, and pituitary function in murine models. Compatible specimen types include mouse serum samples, EDTA plasma preparations, and tissue homogenates prepared from adrenal or pituitary tissue.

Kit contents include a pre-coated microplate, lyophilized ACTH standards, biotinylated detection antibody, streptavidin-HRP conjugate, assay buffer, wash buffer concentrate, TMB substrate, and stop solution. Unused strip wells should be resealed with desiccant to preserve antibody activity, and reconstituted reagents stored refrigerated between uses.

Typical research applications include evaluating circulating ACTH concentrations in mouse models of Cushing's syndrome, Addison's disease, depression, or chronic stress exposure, as well as monitoring HPA axis activity following pharmacological intervention or behavioral manipulation.