Mouse Adrenergic Receptor Beta 2 (ADRb2) ELISA Kit

Beta-2 adrenergic receptor (ADRb2) is a G-protein-coupled receptor that mediates cellular responses to catecholamines such as epinephrine and norepinephrine, influencing smooth muscle relaxation, bronchodilation, cardiac contractility, and metabolic regulation. The Assay Genie Mouse Adrenergic Receptor Beta 2 (ADRb2) ELISA Kit provides quantitative measurement of this receptor in mouse tissue homogenates, supporting research into GPCR pharmacology, adrenergic signaling, asthma models, and cardiovascular physiology.

Each kit delivers 96 assays on a standard microplate using a two-antibody capture-and-detection immunoassay format with a chromogenic absorbance readout. Reconstituted standards and detection antibody should be aliquoted and stored refrigerated; protect from light and limit freeze-thaw cycles to preserve reagent activity. Pre-coated wells, lyophilized standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, and substrate solution are supplied for complete workflow support.

The protocol accommodates small-volume sample inputs from tissue lysates, including lung, cardiac, and adipose preparations. Researchers studying beta-agonist response, receptor downregulation, or sympathetic nervous system activity in mouse disease models can integrate results from this kit alongside western blot and PCR data. Standard curves generated in each run enable quantitative comparison across biological replicates, with intra-assay and inter-assay precision documented for each lot.