Mouse adam17 (Disintegrin and metalloproteinase domain-containing protein 17) ELISA Kit (MOFI01380)

The Mouse ADAM17 ELISA Kit from Assay Genie targets a type I transmembrane metalloproteinase also called TACE (TNF-alpha converting enzyme), the principal sheddase that releases soluble TNF-alpha from its membrane-bound precursor. Quantifying ADAM17 supports research on inflammatory signaling, epithelial-mesenchymal transition, and EGFR-ligand ectodomain cleavage in murine models.

Supplied as a 96 Assays pack, the kit contains a pre-coated microplate strip plate, detection antibody, enzyme conjugate, wash buffer, substrate, and stop solution. The format is a quantitative in vitro immunoassay applicable to cell culture supernatant, tissue homogenate, and serum-derived research samples. Researchers commonly run a standard curve in parallel with unknowns.

Unopened reagents should be refrigerated and protected from light; allow components to reach room temperature before running the assay. Reconstituted standards are best aliquoted to limit freeze-thaw cycles, and unused strip wells can be returned to the foil pouch with desiccant for the stated shelf life.

Applications include biomarker profiling in sepsis and arthritis mouse models, comparing ADAM17 expression in knockout versus wild-type tissue, and screening pharmacological TACE inhibitors in preclinical studies. For orthogonal validation, results are often confirmed by western blot against the mature and pro-form ADAM17 species.