Mouse Activin AB ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit is designed for the precise measurement of Activin AB levels in mouse samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit uses a colorimetric, double-antibody Sandwich ELISA format and reacts with mouse specimens. Kit components should be stored at 2–8°C for 12 months.

Validated performance includes linearity across serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions. Serum linearity ranges from 86–105% at 1:2, 88–105% at 1:4, and 85–101% at 1:8. EDTA plasma linearity ranges from 92–97% at 1:2, 83–99% at 1:4, and 83–98% at 1:8. Heparin plasma linearity ranges from 93–95% at 1:2, 84–96% at 1:4, and 87–99% at 1:8. Spike-and-recovery testing shows average recovery of 92% in serum (range 87–102%), 96% in EDTA plasma (range 91–103%), and 91% in heparin plasma (range 86–96%).

The workflow covers reagent and plate preparation, a 37°C primary incubation of 90 minutes, biotin-labelled detection antibody incubation of 60 minutes, HRP-streptavidin binding for 30 minutes, TMB colour development in the dark for 10–20 minutes, and absorbance reading at 450 nm after stop solution addition. The kit is listed as a research reagent for studying Activin AB, with the source material describing roles in embryonic development, reproductive function, and immune response modulation, and noting that dysregulation has been linked to conditions such as inflammation, fibrosis, and cancer.