Mouse Activating Transcription Factor 3 (ATF3) ELISA Kit

Intended Research Applications

The Assay Genie Mouse ATF3 ELISA Kit is widely used to quantify activating transcription factor 3 in murine research samples, complementing workflows that probe cellular stress signaling, innate immune activation, and tissue injury responses. Investigators studying nerve axotomy, cardiac ischemia-reperfusion, hepatotoxic insult, neuropathic pain, or solid tumor microenvironments routinely track ATF3 induction as a downstream readout of JNK/ERK and integrated stress response pathways.

Workflow and Compatibility

Sample types compatible with this kit include tissue homogenates prepared in standard lysis buffers and clarified cell lysates. A typical run uses 100 uL of diluted sample per microwell, with biotinylated detection antibody plus streptavidin-HRP signal generation read on a standard microplate reader. Reconstituted standard should be aliquoted and stored frozen at -20 degrees C or below to limit freeze-thaw loss, while unused strip wells remain stable refrigerated at 4 degrees C inside the sealed foil pouch with desiccant.