Mouse ACHE/Acetylcholinesterase ELISA Kit

From Assay Genie, this Mouse Acetylcholinesterase (AChE) Sandwich ELISA Kit enables accurate quantification of acetylcholinesterase levels in mouse samples including serum, plasma, tissue homogenates, cell culture supernatant, cell or tissue lysates, and other liquid samples. Acetylcholinesterase plays a crucial role in neurotransmission by breaking down acetylcholine, and its dysregulation has been implicated in various neurological disorders, making it a valuable biomarker for neurobiology research.

The colorimetric Sandwich ELISA delivers a sensitivity of 0.188 ng/mL with a measuring range of 0.781–50 ng/mL. Linearity values are: serum (n=5) 88-102% at 1:2, 87-104% at 1:4, and 85-95% at 1:8; EDTA plasma (n=5) 84-102%, 82-98%, and 85-101%; heparin plasma (n=5) 82-98%, 82-98%, and 87-98%. Recovery ranges are 86-104% (average 94%) for serum, 95-103% (average 99%) for EDTA plasma, and 87-105% (average 95%) for heparin plasma. The kit is stored at 2-8°C for 12 months.

The protocol includes reagent equilibration to room temperature, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody incubation for 60 minutes, HRP-Streptavidin (SABC) binding for 30 minutes, TMB substrate color development in the dark for 10–20 minutes, and absorbance measurement at 450 nm after addition of stop solution. Sample preparation guidance covers serum, plasma, tissue homogenate, cell culture supernatant, and cell lysate.