Mouse Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) ELISA Kit

A quantitative immunoassay from Assay Genie, the Mouse ACAT1 kit measures Acetyl Coenzyme A Acetyltransferase 1 protein levels in mouse-derived samples. ACAT1 is a mitochondrial thiolase enzyme encoded by the nuclear genome, predominantly expressed in liver, kidney, and steroidogenic tissues, where it catalyzes the reversible condensation of two acetyl-CoA molecules to form acetoacetyl-CoA. This enzymatic step sits at a metabolic branch point between ketogenesis, the mevalonate pathway for cholesterol and isoprenoid biosynthesis, and fatty acid β-oxidation.

Configured for 96 assays, the kit accepts tissue homogenates and cell lysates as input material, supporting studies in metabolic disease models, mitochondrial dysfunction, hepatic lipid handling, and ketone body regulation. Researchers investigating PPAR-α signaling, fasting responses, or ketogenic diet interventions commonly use this type of mitochondrial enzyme assay to track ACAT1 expression changes in murine tissues.

Each kit contains the reagents necessary to perform the assay workflow, including a pre-coated plate, detection antibodies, and standards. The assay is validated for reactivity with mouse samples, making it suited to preclinical studies in rodent models of metabolic syndrome, non-alcoholic fatty liver disease, and inherited beta-ketothiolase deficiency.