Mouse Acetyl-CoA carboxylase 1 (Acaca) ELISA Kit

Designed for the quantitative measurement of mouse acetyl-CoA carboxylase 1 (ACACA), this sandwich ELISA from Assay Genie offers high sensitivity and specificity for research applications. The assay employs a colorimetric detection method and detects both natural and recombinant mouse ACACA. It is for research use only.

The kit has a reported detection range of 31.2-2000 pg/mL and a sensitivity of 15.72 pg/mL. Precision is reported as an intra-assay CV of 7.9% and an inter-assay CV of 10.1%. Linearity has been validated in serum, EDTA plasma, and heparin plasma across serial dilutions of 1:2, 1:4, 1:8, and 1:16, with serum recovery averaging 85% (range 80-91%) and plasma recovery averaging 87% (range 81-93%). Validated sample types include serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

ACACA catalyses the rate-limiting step in the biogenesis of long-chain fatty acids and plays a central role in fatty acid biosynthesis and lipid metabolism. Dysregulation of ACACA has been implicated in metabolic disorders such as obesity, diabetes, and cardiovascular diseases, making the assay a useful tool for researchers investigating metabolic regulation and disease pathogenesis.