Mouse AbCG3 ELISA Kit

Designed for the precise measurement of ABCG3 levels in mouse serum, plasma, and cell culture supernatant, this Sandwich ELISA kit from Assay Genie uses a double-antibody format and is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit is positioned for lipid-related research, as ABCG3 is described as a key protein involved in lipid metabolism and cholesterol transport, with dysregulation implicated in atherosclerosis, hypercholesterolemia, and liver disorders.

The assay has a sensitivity of 0.188 ng/mL and a working range of 0.313–20 ng/mL. Storage is at 2–8°C for 12 months. The associated UniProt entry is Q99P81 (synonym: AbCG3), and the kit is classified within the Signal Transduction research area.

Reported linearity (n = 5) across 1:2 to 1:8 dilutions is 86–104% for serum, 83–100% for EDTA plasma, and 80–96% for heparin plasma. Recovery ranges are 86–98% for serum (average 91%), 88–104% for EDTA plasma (average 98%), and 85–105% for heparin plasma (average 98%). The protocol uses antigen binding at 37°C for 90 minutes, biotinylated detection antibody incubation for 60 minutes, HRP-streptavidin binding for 30 minutes, TMB color development in the dark for 10–20 minutes, and absorbance measurement at 450 nm after addition of stop solution.