Mouse A Disintegrin And Metalloprotease 8 (ADAM8) ELISA Kit

Mouse ADAM8, a membrane-anchored metalloprotease involved in ectodomain shedding and extracellular matrix remodeling, can be quantified in tissue homogenates and related biological specimens using this 96-assay immunoassay from Assay Genie. The kit supports research-use-only workflows in laboratories studying inflammatory disease, oncology, and bone biology in murine models.

ADAM8 (also known as CD156a) is a member of the ADAM family that participates in cell adhesion, leukocyte transmigration, and protease-mediated release of surface proteins. It is expressed in macrophages, neutrophils, and other immune cell types, and elevated levels have been documented in mouse models of allergic airway inflammation, osteoclast differentiation, and tumor progression. Researchers investigating these processes use the kit to compare ADAM8 concentrations across normal versus diseased cohorts, with downstream applications including Western blot validation, immunohistochemistry correlation, and cell culture supernatant profiling.

Each kit includes pre-coated microplate strips, assay diluent, calibrated standards, detection antibody, and substrate components needed to complete a run. Working reagents should be aliquoted after reconstitution to limit freeze-thaw cycles, and components should be handled according to standard immunoassay practices for laboratory research.

Typical downstream analyses include correlation with inflammatory cytokines, characterization of protease-substrate relationships, and profiling of transgenic or knockout mouse lines. The Mouse ADAM8 ELISA Kit from Assay Genie fits into broader pipelines that combine ELISA readout with flow cytometry panels, enabling multi-parameter assessment of ADAM8 expression in murine samples.