Mouse 78 kDa glucose-regulated protein (Hspa5) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit enables quantitative measurement of HSPA5 in mouse samples. HSPA5 (also known as Grp78) is an endoplasmic reticulum chaperone central to the cellular stress response, protein folding, and quality control, and plays a crucial role in maintaining cellular homeostasis. The kit is intended for research use only and supports investigation into diseases such as cancer, diabetes, and neurodegenerative disorders.

The assay has a detection range of 78-5000pg/mL and a sensitivity of 39.9pg/mL. Reported precision values are 5.1% intra-assay CV and 7.7% inter-assay CV. It is reactive with mouse serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, and detects natural and recombinant mouse Endoplasmic reticulum chaperone BiP. The target carries the synonyms 78 kDa glucose-regulated protein, Binding-immunoglobulin protein, Heat shock protein 70 family protein 5, Heat shock protein family A member 5, Immunoglobulin heavy chain-binding protein, GRP-78, BiP, HSP70 family protein 5, and Grp78.

Functionally, HSPA5 acts within the endoplasmic reticulum lumen as a key repressor of the ERN1/IRE1-mediated unfolded protein response, participates in the correct folding of proteins and degradation of misfolded proteins via interaction with DNAJC10/ERdj5, and plays an auxiliary role in the post-translational transport of small presecretory proteins across the ER. Listed research areas for the kit include neurosciences.