Mouse 5-hydroxytryptamine ELISA Kit (MOFI01446)

Built around a competitive inhibition format with antigen-coated wells, this Assay Genie kit quantifies serotonin in mouse serum, plasma, cell culture supernatant, and cell or tissue lysate using a 2-hour colorimetric protocol. Manufacturer documentation states that the antibody specifically recognises 5-hydroxytryptamine and shows no obvious cross-reaction with other analogues, supporting clean readouts in monoamine pathway studies where chemically similar molecules are abundant. A 50 uL sample volume is recommended for cell culture supernatant, tissue lysate, and other liquid samples, while only 3 uL of serum or plasma is required per well, an economy that matters when working with limited mouse volumes. The 96-well microplate format includes sealed components that should be refrigerated at 2-8 °C and never frozen, and the kit is intended for research use only in applications such as SSRI pharmacology, transgenic mouse phenotyping, and gastrointestinal or behavioral neuroscience where circulating and tissue serotonin levels are the primary readout.