Mouse 5'-AMP-activated protein kinase catalytic subunit alpha-2 (Prkaa2) ELISA Kit

This Sandwich ELISA kit from Assay Genie is intended for the quantitative detection of mouse 5'-AMP-activated protein kinase catalytic subunit alpha-2 (Prkaa2). PRKAA2 is the catalytic subunit of AMP-activated protein kinase (AMPK), an energy-sensing kinase involved in the regulation of cellular energy metabolism.

The assay reports a detection range of 78-5000pg/mL and a sensitivity of 39.6pg/mL. Intra-assay CV is given as 8.2% and inter-assay CV as 9.7%. The kit is for use with serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, with stated specificity for natural and recombinant mouse PRKAA2. Listed synonyms for the target include Acetyl-CoA carboxylase kinase, Hydroxymethylglutaryl-CoA reductase kinase, ACACA kinase, HMGCR kinase and AMPK subunit alpha-2.

Average recovery is reported at 109% (range 103-115%) for serum and 111% (range 105-117%) for plasma. The associated UniProt accession is Q8BRK8, and PRKAA2 is localised to the cytoplasm and nucleus. The listed research area is cardiovascular. For research use only.