Mouse 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 (Plcg1) ELISA Kit

From Assay Genie, this Sandwich ELISA kit is intended for the quantitative measurement of mouse 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 (Plcg1), also known as Phosphoinositide phospholipase C-gamma-1, Phospholipase C-gamma-1, PLC-gamma-1 or Plcg-1. The assay is reactive with mouse samples and has been validated for use with serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, showing specificity for the natural and recombinant forms of the target.

PLCG1 is a calcium-dependent phosphatidylinositol-specific phospholipase C that mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). It plays an important role in the regulation of intracellular signaling cascades, becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, and contributes to actin reorganization and cell migration. Dysregulation of PLCG1 has been implicated in cancer, immune disorders and neurological conditions, making it a valuable biomarker for disease research. The associated UniProt entry is Q62077 (NCBI Gene ID 18803, protein family: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase), and the highlighted research area is cardiovascular.

Validated performance characteristics include a detection range of 0.156-10ng/ml, a sensitivity of 0.084ng/mL, an intra-assay CV of 6.1% and an inter-assay CV of 8.3%. Linearity has been demonstrated in serum, EDTA plasma and heparin plasma across 1:2, 1:4, 1:8 and 1:16 dilutions, and recovery averaged 100% (range 94-106%) in serum and 102% (range 96-108%) in plasma. The kit is for research use only.