Monkey RANTES/CCL5 ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the accurate measurement of RANTES (CCL5) levels in monkey samples. RANTES (CCL5) is a key chemokine involved in inflammatory responses, recruiting immune cells to sites of infection or inflammation. It plays a critical role in immune regulation and is implicated in diseases such as HIV/AIDS, inflammatory disorders, and allergic reactions, making it a useful target for studying immune responses and inflammatory processes in non-human primate models.

The assay uses a Sandwich ELISA, Double Antibody format with colorimetric detection. It is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Sensitivity is 18.75 pg/mL, with a measuring range of 31.25-2000 pg/mL. The kit should be stored at 2-8°C for 12 months. The associated UniProt identifier is G7PU47, and the target is also known by the synonyms Rantes, CCL5, SCYA5, and SISd.

Validated linearity across serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, and 1:8 ranges from 85% to 105%. Recovery spans 85-99% across the same sample types, with average recoveries of 91-93%. The assay procedure involves primary incubation at 37°C for 90 minutes, detection antibody binding for 60 minutes, HRP-Streptavidin binding for 30 minutes, TMB color development in the dark for 10-20 minutes, and absorbance measurement at 450 nm after stop solution addition.