Monkey IL-1 ELISA Kit (MKFI00102)

From Assay Genie, this Sandwich ELISA kit is configured for the quantitative determination of IL-1 in monkey-derived samples. It uses a double-antibody Sandwich ELISA format with colorimetric detection and accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types as input.

The manufacturer's stated sensitivity is 2.344 pg/mL with a working range of 3.906-250 pg/mL. Reported recovery values across n=5 samples are 85-105% (average 95%) for serum, 91-105% (average 98%) for EDTA plasma, and 85-104% (average 90%) for heparin plasma. Linearity across 1:2, 1:4 and 1:8 dilutions spans 85-105% for serum, 87-104% for EDTA plasma, and 85-105% for heparin plasma. Reagents are stored at 2-8°C for 12 months, per the manufacturer's instructions.

The workflow consists of reagent equilibration to room temperature, a 90-minute primary incubation at 37°C to allow antigen binding, a 60-minute biotin-labeled detection antibody step, a 30-minute HRP-streptavidin (SABC) incubation, 10-20 minutes of TMB color development in the dark, and absorbance measurement at 450 nm after addition of stop solution. Interleukin-1 is described by the manufacturer as a cytokine central to the inflammatory response and immune regulation, with elevated levels implicated in autoimmune disorders, infectious diseases and inflammatory conditions.