Monkey Complement C4/C4 ELISA Kit

A sandwich double-antibody ELISA from Assay Genie developed for the quantitative measurement of complement C4 in monkey samples. The colorimetric assay is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and is suited to research into the complement system and its involvement in immune responses and inflammatory or autoimmune disease.

According to the manufacturer's data, the kit has a sensitivity of 28.125 ng/mL and an assay range of 46.875-3000 ng/mL. Reported recovery values are 89-105% (94% average) for serum, 91-105% (97% average) for EDTA plasma, and 89-100% (94% average) for heparin plasma. Linearity at 1:2, 1:4 and 1:8 dilutions (n=5) is 87-105%, 88-98% and 80-95% for serum; 86-101%, 87-101% and 80-99% for EDTA plasma; and 85-105%, 83-101% and 80-92% for heparin plasma. The protocol involves a 90-minute primary incubation at 37°C, a 60-minute biotinylated detection antibody step, 30 minutes of HRP-streptavidin binding, and TMB colour development for 10-20 minutes in the dark, with absorbance read at 450 nm.

Kit components include a pre-coated dismountable microplate, lyophilized standard, 100X biotin-labelled antibody, 100X HRP-streptavidin conjugate (SABC), TMB substrate, sample and antibody dilution buffers, SABC dilution buffer, stop solution, 25X wash buffer, plate sealers and a technical manual. The kit should be stored at 2-8°C for 12 months.