Monkey C5a/Complement C5a ELISA Kit

A Sandwich ELISA kit from Assay Genie designed for the quantitative measurement of C5a complement factor levels in monkey samples. The assay is intended for research applications in immunology and inflammation, where C5a is described as a key component of the innate immune response with a critical role in inflammation and host defense against pathogens. The kit is positioned as a tool for studying C5a dysregulation, which the source associates with various inflammatory diseases and as a target for therapeutic interventions and biomarker studies.

Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit is intended to be stored at 2-8°C for 12 months.

The procedure involves equilibrating reagents and TMB substrate, a primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding at 37°C for 60 minutes, HRP-Streptavidin (SABC) incubation at 37°C for 30 minutes, TMB color development in the dark for 10–20 minutes, and a stop reaction with absorbance read at 450 nm. Reported linearity across 1:2, 1:4 and 1:8 dilutions spans 80-105% for serum, EDTA plasma, and heparin plasma, and reported average recovery ranges from 93-97% across the same sample types.