Monkey Adiponectin Receptor 1/ADIPOR1 ELISA Kit

Built for the accurate detection of Adiponectin Receptor 1 (ADIPOR1) levels in monkey samples, this Sandwich, Double Antibody ELISA from Assay Genie is intended for research applications. The assay accommodates serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. ADIPOR1 is a critical receptor involved in adiponectin signaling, which regulates glucose and fatty acid metabolism, and its dysregulation has been linked to obesity, diabetes, and cardiovascular diseases, making it a relevant biomarker for metabolic research.

Key specifications include a sensitivity of 0.188 ng/mL and a detection range of 0.313-20 ng/mL. Linearity testing showed consistent results across serum (85-104%, 85-103%, 85-103%), EDTA plasma (83-98%, 87-94%, 82-101%), and heparin plasma (85-95%, 85-93%, 86-96%) at 1:2, 1:4, and 1:8 dilutions (n = 5). Recovery ranged from 89-105%, with averages of 95% (serum), 99% (EDTA plasma), and 93% (heparin plasma). The kit is stored at 2-8°C for 12 months.

The workflow involves reagent and plate preparation, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding, HRP-Streptavidin incubation, TMB substrate color development in the dark for 10–20 minutes, and absorbance reading at 450 nm after adding stop solution. Synonyms for ADIPOR1 include ACDCR1, CGI45, PAQR1, and TESBP1A (Uniprot: A0A0D9RP26).