MNK1 (Phospho-Thr255)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit is specifically designed for the quantitative detection of phosphorylated MNK1 at threonine 255 in cell lysates. The assay provides a lysate-free, high-throughput format suitable for adherent cells, allowing researchers to measure MNK1 protein phosphorylation and expression profiles and to screen the effects of various treatments, inhibitors, or activators on MNK1 phosphorylation.

The kit uses an indirect ELISA format in which MNK1 (Phospho-Thr255) is captured by phospho-Thr255-specific primary antibodies, with HRP-conjugated secondary antibodies enabling a colorimetric reaction upon substrate addition. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalization, and Crystal Violet whole-cell staining can be used to determine cell density. Reactivity covers Human, Mouse, and Rat, with a dynamic range of > 5000 Cells and detection at 450 nm. MNK1 (gene symbol MKNK1; UniProt Q9BUB5; Gene ID 8569; molecular weight 39,104 Da) is a Ser/Thr protein kinase that regulates translation, and phosphorylation at threonine 255 influences its kinase activity, making it relevant to research on cell signaling and molecular biology.