MN1 Colorimetric Cell-Based ELISA

This Cell-Based ELISA Kit from Assay Genie is designed for the qualitative detection and relative quantification of MN1 protein expression in cultured cells. It uses an indirect ELISA format: MN1-specific primary antibodies capture the target, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, allowing the conjugated HRP to catalyze a colorimetric reaction upon substrate addition. The kit is described as lysate-free and convenient, and can be used both for measuring relative MN1 levels in cultured cells and for screening the effects of treatments, inhibitors (such as siRNA or chemicals) or activators on MN1 expression.

Detection is colorimetric, read at 450 nm, with a stated dynamic range of > 5000 Cells. The assay is reactive with human samples and is suited to adherent cells, and a monoclonal anti-GAPDH antibody is included as an internal positive control to normalize target absorbance values. Following the colorimetric read, an optional Crystal Violet whole-cell staining step may be used to determine cell density so that absorbance values can be further normalized to cell amount. The kit is supplied in a 96-Well Microplate format, stored at 4 °C with a stated stability of 6 Months, and is classified within the Epigenetics and Nuclear Signaling research area.

MN1 is described as a key regulator of cell proliferation and differentiation, with dysregulation implicated in multiple diseases including leukemia and solid tumors, making it a target of interest for studies of disease mechanisms and potential therapeutic interventions. According to the UniProt annotation provided, MN1 may play a role in tumor suppression; chromosomal aberrations involving MN1 have been associated with acute myeloid leukemia, and defects in MN1 have been linked to the development of meningiomas.