MMP-8 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based colorimetric immunoassay is designed for the detection of MMP-8, also known as Collagenase-2. MMP-8 is a key enzyme involved in the breakdown of extracellular matrix proteins, making it crucial in processes such as tissue remodeling and wound healing. Dysregulation of MMP-8 activity has been linked to diseases including arthritis, cancer, and cardiovascular disorders, highlighting its importance as a potential biomarker for disease progression and therapeutic development.

The kit uses an indirect ELISA format in a 96-Well Microplate, with colorimetric detection read at 450 nm. It is a convenient, lysate-free, high-throughput and sensitive assay that detects the MMP-8 protein expression profile in cultured cells. It enables qualitative determination of MMP-8 in cell samples and can be used to screen the effects of various treatments, inhibitors, or activators. The assay captures MMP-8 with target-specific primary antibodies; HRP-conjugated secondary antibodies bind the Fc region of the primary antibody and catalyze a colorimetric reaction upon substrate addition.

Sample type is adherent cells, with reactivity reported for human, mouse, and rat. Normalization is supported by an included monoclonal antibody specific for human GAPDH as an internal positive control, along with a Crystal Violet whole-cell staining method to determine cell density. The dynamic range is stated as > 5000 Cells, and storage is at 4°C for 6 Months.