MKK6 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit provides a qualitative, lysate-free method for measuring MKK6 protein expression profiles in cultured adherent cells. Detection is colorimetric, with absorbance read at 450 nm, and the kit is reactive with Human, Mouse, and Rat samples. It is suited to high-throughput workflows and to screening the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on MKK6 levels.

The assay uses an indirect ELISA format: MKK6 is captured by target-specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colour reaction upon substrate addition. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalising target absorbance, and Crystal Violet whole-cell staining is provided to determine cell density for further normalisation.

MKK6 (gene symbol MAP2K6, Gene ID 5608, UniProt P52564) is a dual specificity protein kinase and an essential component of the MAP kinase signal transduction pathway. The stated dynamic range is > 5000 cells, and the kit is stored at 4 °C with a stability of 6 months.