MITF Colorimetric Cell-Based ELISA Kit

Assay Genie's cell-based ELISA enables colourimetric detection of microphthalmia-associated transcription factor (MITF) in cultured adherent cells, without the need for lysate preparation. The assay uses an indirect ELISA format in which MITF is bound by target-specific primary antibodies and detected using HRP-conjugated secondary antibodies; signal is generated colourimetrically at 450 nm upon substrate addition. Reactivity is established for Human and Mouse samples, with a stated dynamic range of > 5000 Cells.

Two normalisation approaches are supplied with the kit. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising target absorbance values. After the HRP colourimetric readout, the Crystal Violet whole-cell staining method may be used to determine cell density, allowing absorbance values to be normalised to cell amounts and adjusting for plating variation. The kit is stored at 4°C for 6 months and is provided in a 96-well microplate format.

MITF is a transcription factor containing both basic helix-loop-helix and leucine zipper structural features, and it plays a critical role in the differentiation of neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. The kit supports research applications in melanocyte biology, skin pigmentation, melanoma and related skin disorders, and can be used to screen the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on MITF expression in cultured cells.