mGluR7 Colorimetric Cell-Based ELISA Kit

Assay Genie's cell-based ELISA kit provides researchers with a sensitive, lysate-free method for measuring metabotropic glutamate receptor 7 (mGluR7) protein expression directly in cultured cells. The kit supports high-throughput screening of treatments, inhibitors such as siRNA or chemicals, and activators, allowing assessment of receptor activity in cell models and aiding studies of mGluR7 signaling pathways relevant to neurological disorders.

The assay uses a colorimetric detection method measured at 450 nm and is suitable for adherent cells. It shows reactivity with Human, Mouse, and Rat samples, with a dynamic range of > 5000 Cells. The kit is provided in a 96-Well Microplate format and is stored at 4°C with a stability of 6 months.

Working on an indirect ELISA principle, the target is captured by mGluR7-specific primary antibodies, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody to catalyze a colorimetric reaction upon substrate addition. A monoclonal anti-GAPDH antibody is included to serve as an internal positive control for normalizing target absorbance values. Following colorimetric measurement of HRP activity, the Crystal Violet whole-cell staining method can be used to determine cell density, allowing absorbance values to be normalized to cell amounts to adjust for plating differences.