mGluR4 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit is designed for the accurate detection of metabotropic glutamate receptor 4 (mGluR4) levels in cell samples, with the manufacturer describing it as offering exceptional sensitivity and specificity, a user-friendly protocol and robust performance.

It is a convenient, lysate-free, high-throughput and sensitive workflow for measuring the relative amounts of mGluR4 in cultured cells, and for screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on mGluR4. The assay is qualitative, using an indirect ELISA format in which mGluR4 is captured by target-specific primary antibodies and detected via HRP-conjugated secondary antibodies that catalyse a colorimetric reaction. Reactivity is confirmed against human, mouse and rat samples, with adherent cells as the validated sample type. Detection is colorimetric, read at 450 nm, with a stated dynamic range of > 5000 Cells. Storage is 4°C for 6 Months. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalisation, and a Crystal Violet whole-cell staining method may be used to determine cell density.

mGluR4 is a G-protein coupled receptor for glutamate that regulates synaptic transmission and neuronal signalling, and its dysregulation has been implicated in conditions such as Parkinson's disease, schizophrenia and addiction, making this kit a useful tool for basic research and drug discovery studies.