Methylmalonic Acid (MMA) ELISA Kit

From Assay Genie, this Competitive ELISA Kit is designed for the precise measurement of methylmalonic acid (MMA) levels in biological samples such as serum, plasma, and urine. The assay works across a working range of 0.312–20 ng/mL, supporting research applications where MMA is investigated as a metabolite associated with vitamin B12 deficiency and various metabolic disorders. Abnormal MMA concentrations may be linked to conditions including anemia, kidney disease, and certain neurological disorders, making the kit a useful tool for studies of disease pathogenesis and treatment monitoring.

The manufacturer supplies protocols and guidance for handling serum, plasma, urine, cerebrospinal fluid, cell culture supernatants, cell lysates, tissue homogenates, tissue lysates, and breast milk, with notes to avoid multiple freeze-thaw cycles and to exclude over-haemolysed samples. Optical density is read at 450 nm using a microplate reader, following incubation steps with Detection Reagent A and B and a substrate solution, stopped with the provided Stop Solution. The kit is intended for research use only.