Met (Phospho-Tyr1234)Colorimetric Cell-Based ELISA Kit

Assay Genie offers a Cell-Based ELISA Kit designed for the specific detection of Met phosphorylated at tyrosine 1234, a key modification on a receptor tyrosine kinase involved in cell proliferation, survival, and migration. The kit uses a colorimetric detection method measured at 450 nm and is reactive with Human, Mouse, and Rat samples, making it a versatile tool for cell signaling research.

The assay employs a lysate-free, indirect ELISA format in which phospho-specific primary antibodies capture Met (Phospho-Tyr1234), and HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control for normalizing target absorbance values, and the Crystal Violet whole-cell staining method may be used to determine cell density. The assay has a dynamic range of > 5000 Cells, with adherent cells as the primary sample type.

By measuring relative levels of phosphorylated Met in cultured cells, researchers can study the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on Met phosphorylation. The target is associated with the MET gene (UniProt ID: P08581), and Met phosphorylation at tyrosine 1234 is known to be involved in signaling pathways related to cancer progression, metastasis, and drug resistance, supporting applications in cancer biology and drug discovery research.