MEK1 (Phospho-Thr291)Colorimetric Cell-Based ELISA Kit

Assay Genie offers a Cell-Based ELISA Kit designed for the qualitative measurement of MEK1 phosphorylated at Thr291 in cultured cell samples. The kit uses a colorimetric detection method and is intended for use with adherent cells, providing a lysate-free, high-throughput workflow suited to studies of cellular signaling within the MAPK pathway.

Reactivity is established for human, mouse, and rat samples, with a dynamic range of more than 5000 cells. The assay employs an indirect ELISA format in which a phospho-specific primary antibody captures the target and an HRP-conjugated secondary antibody drives a colorimetric reaction upon substrate addition. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalization, and a Crystal Violet whole-cell stain is provided to determine cell density after the colorimetric read.

Designed to detect MEK1 protein phosphorylation and expression profile, this kit is suited to evaluating the relative levels of phosphorylated MEK1 in response to treatments, inhibitors, or activators, supporting research into signal transduction pathways and potential therapeutic targets.