MEK1 (Phospho-Ser298)Colorimetric Cell-Based ELISA Kit

This Cell-Based ELISA Kit from Assay Genie is designed for the accurate detection of phosphorylated MEK1 at serine 298 in cell lysates. It offers high specificity and delivers reliable, reproducible results for research applications related to the MAPK signalling pathway, where phosphorylation of MEK1 at serine 298 regulates its activity and plays a crucial role in cell growth, differentiation, and survival. Dysregulation of this pathway is implicated in various diseases, including cancer and inflammatory disorders.

The assay uses a convenient, lysate-free, indirect ELISA format in which MEK1 (Phospho-Ser298) is captured by phospho-specific primary antibodies and detected via HRP-conjugated secondary antibodies, producing a colorimetric readout at 450 nm. It enables measurement of relative phosphorylation in cultured cells and screening of the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators. A monoclonal anti-GAPDH antibody is included as an internal positive control, and a Crystal Violet whole-cell staining method supports normalisation of absorbance to cell density. Reactivity covers Human, Mouse, and Rat samples, with a dynamic range of > 5000 cells.