MEK1/2 (Phospho-Ser221)Colorimetric Cell-Based ELISA Kit

This colorimetric cell-based ELISA kit from Assay Genie is designed for the quantitative measurement of MEK1/2 phosphorylation at serine 221 in cell lysates. The assay uses an indirect ELISA format in which the target is captured by phospho-specific primary antibodies, with HRP-conjugated secondary antibodies catalysing a colorimetric reaction upon substrate addition. The protocol is lysate-free and high-throughput, and is intended for use with adherent cells.

Reactivity has been demonstrated against human, mouse and rat samples, with a quoted dynamic range of > 5000 cells and detection at 450 nm. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining step may be used to determine cell density and adjust for plating differences.

The kit is intended for measuring the relative amounts of phosphorylated MEK1/2 in cultured cells and for screening the effects of various treatments, inhibitors (such as siRNA or chemicals) or activators on MEK1/2 phosphorylation. The associated gene symbols are MAP2K1/MAP2K2 and the sub-type is Phospho, with gene IDs 5604/5605 and UniProt IDs Q02750/P36507.