MEK1/2 (Phospho-Ser217)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed for the qualitative detection of MEK1/2 phosphorylated at serine 217, supporting research into MAPK signaling pathway activation. It uses a colorimetric detection method measured at 450 nm and is validated for use with human, mouse, and rat samples, with a dynamic range of "> 5000 Cells." The assay works in an indirect ELISA format in which a target-specific primary antibody captures phosphorylated MEK1/2 (Phospho-Ser217) in cultured cells, and an HRP-conjugated secondary antibody binds the Fc region of the primary antibody to catalyze a colorimetric reaction upon substrate addition.

Described as lysate-free and suited to high-throughput applications, the kit can be used to measure the relative amounts of phosphorylated MEK1/2 in cultured cells and to screen the effects of various treatments, siRNA, or chemical inhibitors and activators on MEK1/2 phosphorylation. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining step can be used to determine cell density and adjust for plating differences. The target corresponds to the MAP2K1/MAP2K2 gene products (UniProt Q02750/P36507), and the sub-type is phospho-specific.