MEK1/2 Colorimetric Cell-Based ELISA Kit

This cell-based In-Cell ELISA kit from Assay Genie is designed for the detection and measurement of MEK1/2 protein expression in cultured cells. Using a colorimetric detection method at 450 nm in an indirect ELISA format, the assay captures MEK1/2 with target-specific primary antibodies while HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition. The kit is lysate-free and configured in a 96-well microplate format suitable for high-throughput use with adherent cell samples.

The kit has a stated dynamic range of > 5000 cells and is reactive with Human, Mouse, and Rat samples. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining step is provided to determine cell density so that absorbance values can be normalized to cell amounts, correcting for plating differences.

MEK1 and MEK2 (MAP2K1/MAP2K2) are dual-specificity protein kinases that sit upstream in the MAPK/ERK signal transduction cascade, regulating processes such as proliferation, differentiation, transcription regulation and development, and have been linked to cancer, inflammatory disorders and neurological disorders. The kit is intended for researchers studying MAPK signaling pathways, and can be used to measure relative MEK1/2 levels in cultured cells or to screen the effects of treatments, inhibitors (such as siRNA or chemicals) and activators. It should be stored at 4°C with a stated stability of 6 Months.