MEF2A (Phospho-Thr319)Colorimetric Cell-Based ELISA Kit

Assay Genie's cell-based ELISA kit is designed for the quantitative detection of phosphorylated MEF2A (myocyte enhancer factor 2A) at threonine 319 in cells. The assay provides a convenient, lysate-free, high-throughput and sensitive workflow for measuring the relative amounts of phosphorylated MEF2A in cultured cells, and is suited to screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on MEF2A phosphorylation.

Detection is performed via an indirect ELISA format, in which phospho-specific primary antibodies capture the target and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. The conjugated HRP catalyses a colorimetric reaction read at 450 nm, with a dynamic range of > 5000 Cells. Reactivity is established for Human, Mouse and Rat samples, and the kit is intended for use with adherent cells. For normalisation, a monoclonal antibody specific for human GAPDH is included as an internal positive control, and Crystal Violet whole-cell staining may be used to determine cell density, allowing absorbance values to be normalised to cell numbers.

MEF2A is a transcription factor that regulates gene expression in response to cellular signals; phosphorylation at threonine 319 modulates its activity and influences processes such as cell differentiation, proliferation and survival, making the kit applicable to studies of MEF2A-mediated signalling in contexts including cancer, cardiovascular disorders and neurological conditions.