MCM5 Colorimetric Cell-Based ELISA Kit

A Cell-Based ELISA Kit from Assay Genie designed for the qualitative detection of MCM5 protein expression in cultured cells. The assay uses an indirect ELISA format in which MCM5-specific primary antibodies capture the target and HRP-conjugated secondary antibodies generate a colorimetric signal upon substrate addition, with absorbance read at 450 nm. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values, and Crystal Violet whole-cell staining is provided to determine cell density and adjust for plating differences.

Reactivity is reported for Human, Mouse, and Rat, and the kit is described as suitable for measuring relative MCM5 levels in cell lysates, serum, and plasma, as well as for screening the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on MCM5. The stated dynamic range is greater than 5000 cells, the format is a 96-Well Microplate, the detection method is colorimetric at 450 nm, and storage stability is given as 4 °C for 6 months. The product is positioned for research into DNA replication and cell cycle regulation, including studies of cancer, genetic disorders, and developmental abnormalities.