MCM2 Colorimetric Cell-Based ELISA Kit

Designed for researchers studying DNA replication and cell cycle regulation, this Cell-Based ELISA from Assay Genie provides a high throughput, lysate-free approach to detecting MCM2 protein expression in cultured cells. The kit uses an indirect ELISA format in which MCM2-specific primary antibodies capture the target, while HRP-conjugated secondary antibodies catalyse a colorimetric reaction detected at 450 nm. It is validated for Human and Mouse reactivity with a dynamic range of > 5000 Cells, and supports qualitative measurement of MCM2 levels in cells exposed to treatments, inhibitors (such as siRNA or chemicals), or activators.

A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising target absorbance, and the Crystal Violet whole-cell staining method can subsequently be used to determine cell density and adjust for plating differences. The kit is held at 4 °C and remains stable for 6 Months, and is intended for research in the Cell Cycle area.

MCM2 is a mini-chromosome maintenance protein essential for the initiation of eukaryotic genome replication, required for entry into S phase and for cell division. Dysregulation of MCM2 has been implicated in cancer and developmental disorders, making it a target of interest for therapeutic investigation.