MARCKS Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit is designed for detecting and quantifying Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) levels in cell lysates and culture media, with a recommended sample type of adherent cells. The kit shows reactivity with Human, Mouse, and Rat samples and uses a Colorimetric detection method read at 450 nm, with a stated dynamic range of > 5000 Cells. It is supplied in a 96-Well Microplate format and is intended for storage at 4°C for 6 Months.

The assay is a convenient, lysate-free, high-throughput and sensitive Cell-Based ELISA that provides qualitative determination of MARCKS concentration through an indirect ELISA format. MARCKS is captured by MARCKS-specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition. The kit can be used to measure relative MARCKS levels in cultured cells and to screen the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on MARCKS expression.

Two normalisation methods are built into the workflow. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control for normalising target absorbance values. Following the colorimetric measurement of HRP activity, the Crystal Violet whole-cell staining method may be used to determine cell density, allowing absorbance values to be normalised to cell amounts and adjusting for plating differences.