MAPK3 Colorimetric Cell-Based ELISA

This cell-based ELISA kit from Assay Genie is designed for the accurate and sensitive detection of MAPK3 protein levels directly in cultured cells. MAPK3, also known as ERK1, is a key signalling protein in the MAPK/ERK pathway that regulates cellular processes including cell growth, differentiation, and survival, and its dysregulation has been linked to cancer, inflammatory disorders, and neurodegenerative diseases. The kit provides a convenient, lysate-free, high-throughput format suitable for measuring relative MAPK3 expression in adherent cells and for screening the effects that various treatments, inhibitors, or activators have on MAPK3.

The assay uses an indirect ELISA format: MAPK3 is captured by target-specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining method is provided to determine cell density and adjust for plating differences. Together these steps support qualitative determination of MAPK3 concentration in cells.

The kit is reactive with human, mouse, and rat samples, with a reported dynamic range of > 5000 cells and colorimetric detection at 450 nm. It is supplied in a 96-well microplate format and should be stored at 4 °C, where it remains stable for 6 months.