LysoBrite, Green, 500tests

LysoBrite Green from STEMCELL Technologies is a cell-permeable fluorescent probe specifically formulated to label acidic lysosomal compartments in live cells. The dye accumulates within lysosomes through proton trapping, producing bright green fluorescence suitable for fluorescence microscopy, immunohistochemistry, and flow cytometry workflows. Each vial contains sufficient reagent for 500 tests, supplied as a stable stock solution that researchers dilute directly into growth medium or buffer before adding it to adherent or suspension cultures.

Typical working protocols involve incubating cultured cells with the probe for 15 to 30 minutes at 37 degrees C, followed by washing and live-cell imaging on an inverted microscope or confocal platform. Excitation is efficiently driven by standard 488 nm argon laser lines, with emission captured around 500 to 520 nm. The reagent is stored frozen at -20 degrees C and protected from light to preserve fluorescence intensity through repeated freeze-thaw cycles; small single-use aliquots are recommended to limit handling and photobleaching.

Investigators studying autophagy, endolysosomal trafficking, phagocytosis, or lysosomal storage disorders rely on this probe to visualize organelle morphology, monitor lysosomal pH dynamics, and quantify lysosomal content in flow cytometry panels. The format integrates readily with multi-well plate assays, high-content screening routines, and co-localization studies alongside organelle-specific markers such as MitoTracker or ER-Tracker, making it a versatile reagent for cell biology and research use only applications.