DiOC6(3) Iodide, 25mg

STEMCELL Technologies offers a green fluorescent lipophilic dye used for staining cell membranes and other hydrophobic structures in live and fixed cells. The dye, chemically 3,3'-dihexyloxacarbocyanine iodide (CAS Number: 53213-82-4; Chemical Formula: C29H37IN2O2; Molecular Weight: 572.52 g/mol), is membrane permeable and accumulates in the mitochondria of live cells at low concentrations. At higher concentrations, it additionally stains other membranes of live cells, including the endoplasmic reticulum and the Golgi apparatus, and also stains extracellular vesicles.

The dye has an excitation wavelength of 483 nm and an emission wavelength of 501 nm, and is supplied as a red powder. It should be stored at -20°C, protected from prolonged exposure to light, and remains stable until the expiry date (EXP) on the box label.

For use, a stock solution is prepared in dimethyl sulfoxide (DMSO) or ethanol at 1 - 10 mM, and a working solution is prepared by diluting the stock in a suitable buffer such as phosphate-buffered saline (PBS) to 1 - 10 μM. For cells in suspension, cells are resuspended at 1 x 10^6 cells/mL in working solution and incubated at 37°C for 2 - 20 minutes, protected from light, then centrifuged at 110 - 250 x g for 5 minutes and washed. Detection can be performed by microscopy using a standard FITC filter, or by flow cytometry on the conventional FL1 detection channel.