LIMK1/2 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this in-cell colorimetric ELISA provides qualitative detection of LIMK1/2 protein expression directly in cultured cells. The assay shows reactivity with Human, Mouse and Rat samples and uses a colorimetric readout at 450 nm, with a stated dynamic range of > 5000 Cells. The indicated sample type is adherent cells, and the kit is configured for a 96-well microplate format. Storage is at 4 °C for up to 6 months.

The workflow is lysate-free and high throughput, relying on an indirect ELISA format in which LIMK1/2 is captured by target-specific primary antibodies and detected using HRP-conjugated secondary antibodies that catalyze a colorimetric reaction on substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalisation of target absorbance values. For cell-density normalisation, a Crystal Violet whole-cell staining step can be performed after the HRP readout, allowing absorbance values to be adjusted for differences in cell plating. The kit is also suitable for screening the effects of various treatments, inhibitors (such as siRNA or chemicals) or activators on LIMK1/2 levels in cultured cells.

LIMK1 is a serine/threonine kinase that regulates actin cytoskeleton dynamics and cellular morphology, with described roles in cell migration, invasion and metastasis. Dysregulation of LIMK1 has been implicated in various diseases, including cancer, neurodegenerative disorders and autoimmune conditions, making the kit a relevant tool for research into these areas.