Lck (Phospho-Tyr393)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit enables qualitative detection of phosphorylated Lck at Tyr393 in adherent cultured cells, offering a convenient, lysate-free, high-throughput workflow for studying T-cell receptor signalling and immune cell function. The assay uses an indirect ELISA format, in which a Lck (Phospho-Tyr393)-specific primary antibody captures the target and an HRP-conjugated secondary antibody binds the Fc region of that primary antibody. Upon substrate addition, the conjugated HRP catalyses a colorimetric reaction read at 450 nm. Reactivity is reported for Human, Mouse, and Rat, with a dynamic range of > 5000 Cells.

A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining step may be used to determine cell density for further normalisation of plating differences. Lck is a member of the Src family of tyrosine kinases and a critical regulator of T-cell activation, with phosphorylation at Tyr393 linked to its activation and downstream signalling in T-cell receptor pathways — making the kit relevant to research in immunology, oncology, and drug development.