Lck (Phospho-Tyr192)Colorimetric Cell-Based ELISA Kit

Designed by Assay Genie, this Cell-Based ELISA Kit enables accurate quantification of phosphorylated Tyr192 levels in Lck protein samples from adherent cells. The colorimetric assay uses a lysate-free, high-throughput workflow suited to measuring relative Lck phosphorylation in cultured cells, as well as screening the effects of various treatments, inhibitors (such as siRNA or chemicals), and activators on Lck phosphorylation.

Detection is achieved through an indirect ELISA format in which Lck (Phospho-Tyr192) is captured by a specific primary antibody and detected with an HRP-conjugated secondary antibody, producing a colorimetric readout at 450 nm. The kit is reactive with human, mouse, and rat samples and has a dynamic range of > 5000 Cells. To support normalisation, a monoclonal antibody specific for human GAPDH is included as an internal positive control, and a Crystal Violet whole-cell staining step can be used to determine cell density and adjust for plating differences.

Lck is a Src-family tyrosine kinase critical to T-cell antigen receptor signalling, and phosphorylation of Tyr192 is reported to regulate its activity, making this kit a useful tool for studies of immune signalling, T-cell activation, and related disease research (gene symbol LCK, UniProt P06239).