LAT3 Colorimetric Cell-Based ELISA

This cell-based ELISA kit from Assay Genie is designed for the precise measurement of LAT3 (L-type Amino Acid Transporter 3) levels in cell lysates and tissue homogenates. The assay targets LAT3, a key transporter protein responsible for the uptake of essential amino acids in cells, and is intended for use with human samples in signal transduction research. Dysregulation of LAT3 has been implicated in cancer, metabolic disorders, and neurological conditions, making it a valuable target for investigation and drug development.

The kit uses a lysate-free, high throughput and sensitive format to detect LAT3 protein expression profiles in cultured cells. It can be used to measure the relative amounts of LAT3 as well as to screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators. Qualitative determination of LAT3 concentration is achieved by an indirect ELISA format in which LAT3-specific primary antibodies capture the target, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition. The dynamic range is greater than 5000 cells, with detection carried out colorimetrically at 450 nm.

Normalisation is supported by an included monoclonal antibody specific for human GAPDH, which serves as an internal positive control, and by an optional Crystal Violet whole-cell staining method for determining cell density. The kit is provided in a 96-well microplate format and is intended for adherent cell samples, with storage at 4°C for 6 months.