LAT (Phospho-Tyr191)Colorimetric Cell-Based ELISA Kit

Designed for the detection and quantification of phosphorylated LAT (Linker for Activation of T cells) at tyrosine 191, this Cell-Based ELISA Kit from Assay Genie offers a lysate-free, high-throughput workflow for measuring relative phosphorylation levels directly in cultured cells. The assay uses an indirect ELISA format, in which LAT (Phospho-Tyr191) is captured by phospho-specific primary antibodies and detected with HRP-conjugated secondary antibodies that catalyse a colorimetric reaction read at 450 nm.

Reactivity is reported for Human, Mouse and Rat samples, with a dynamic range of > 5000 Cells. A monoclonal anti-GAPDH antibody is included to serve as an internal positive control for normalising target absorbance values, and the Crystal Violet whole-cell staining method can be applied to determine cell density for further normalisation. The kit is intended for research in immunology and is suitable for screening the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on LAT phosphorylation.

Compatible with adherent cell samples, the assay is supplied in a 96-well microplate format and includes the phospho-specific anti-LAT (Phospho-Tyr191) antibody, an anti-LAT (total) antibody, anti-GAPDH antibody, HRP-conjugated anti-rabbit and anti-mouse IgG secondary antibodies, wash and blocking buffers, substrate, stop solution and Crystal Violet Solution for cell density measurement.