LAT (Phospho-Tyr171)Colorimetric Cell-Based ELISA Kit

A colorimetric cell-based ELISA kit from Assay Genie is designed to measure the level of phosphorylated LAT (Linker for Activation of T cells) at tyrosine 171 in cell lysates or tissue samples. The assay uses an indirect ELISA format in which LAT (Phospho-Tyr171) is captured by specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody to catalyze a colorimetric reaction upon substrate addition.

The kit is a lysate-free, high-throughput and sensitive assay that can detect LAT protein phosphorylation and expression profiles in cells, and is suitable for screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on LAT phosphorylation. Detection is read at 450 nm with a dynamic range of > 5000 cells, and reactivity covers human, mouse, and rat samples. The intended research application area is immunology, supporting studies of T cell receptor signaling, immune cell activation, and related disorders.

A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, and the Crystal Violet whole-cell staining method may be used to determine cell density so that absorbance values can be normalized to cell amounts and plating differences corrected.