Lamin A/C (Phospho-Ser392)Colorimetric Cell-Based ELISA Kit

This colorimetric cell-based ELISA kit from Assay Genie is designed for the detection of phosphorylated Lamin A/C at Ser392 in adherent cell samples. The assay uses a qualitative indirect ELISA format in which phospho-specific primary antibodies capture the target and HRP-conjugated secondary antibodies catalyze a colorimetric reaction measured at 450 nm.

Reactive with human, mouse, and rat samples, the kit covers a dynamic range of more than 5000 cells and is described as a lysate-free, high-throughput, and sensitive workflow. It can be used to measure relative phosphorylation levels in cultured cells and to screen the effects of various treatments, inhibitors (such as siRNA or chemicals), and activators on Lamin A/C phosphorylation.

A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control for normalizing target absorbance values, and the Crystal Violet whole-cell staining method may be used to determine cell density so that absorbance values can be adjusted for plating differences. The target protein is encoded by the LMNA gene.