Ku70/XRCC6 (Phospho-Ser5)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit is designed for the qualitative detection of phosphorylated KU70 (XRCC6) at serine 5 in cultured cells. The assay uses a colorimetric detection method measured at 450 nm and an indirect ELISA format, in which Ku70/XRCC6 (Phospho-Ser5) is captured by target-specific primary antibodies and HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition.

Phosphorylation of KU70 (XRCC6) at serine 5 is implicated in DNA damage response and repair pathways, influencing the cellular response to genotoxic stress and contributing to genomic stability. The kit is suited to measuring the relative amounts of phosphorylated Ku70/XRCC6 in cultured cells, as well as screening for the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on Ku70/XRCC phosphorylation. It is reactive with human and mouse samples, with a dynamic range of > 5000 cells. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalization, and Crystal Violet whole-cell staining may be used to determine cell density after the colorimetric measurement.

The research area is Epigenetics and Nuclear Signaling, and the indicated sample type is adherent cells.